Translucent teal and coral tissue ribbons weave around immune and tumour cells, connected by delicate amber metabolic particles.

Publications.

Selected publications

Oval detail of the Dhps-WT lung fluorescence image from Figure 4k, showing macrophages among surrounding stromal cells. Open the full figure for labels and scale.

Nature2026

The transition from monocyte to tissue-resident macrophage requires DHPS

Gustavo E. Carrizo, Pianpian Lin, Seung Hyun Lee, …, David E. Sanin, Daniel J. Puleston^*, Erika L. Pearce^*

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Gustavo E. Carrizo, Pianpian Lin, Seung Hyun Lee, Kevin Shenderov, Camille Blériot, Minsun Cha, Lena Schimmelpfennig, Zhen Shen, Nikki van Teijlingen Bakker, Katarzyna M. Grzes, Beth Kelly, Niloufar Safinia, Kate L. Schole, Yaarub Musa, Gerhard Mittler, Yoh Zen, Edward J. Pearce, Florent Ginhoux, David E. Sanin, Daniel J. Puleston^*, Erika L. Pearce^*

Published graphical abstract comparing normal polyamine metabolism with polyamine deficiency and their effects on helper T-cell epigenetic programming and lineage commitment.

Cell2021

Polyamine metabolism is a central determinant of helper T cell lineage fidelity

Daniel J. Puleston, Francesc Baixauli, David E. Sanin, …, Edward J. Pearce, Douglas R. Green, Erika L. Pearce^*

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Daniel J. Puleston, Francesc Baixauli, David E. Sanin, Joy Edwards-Hicks, Matteo Villa, Agnieszka M. Kabat, Marcin M. Kamiński, Michal Stanckzak, Hauke J. Weiss, Katarzyna M. Grzes, Klara Piletic, Cameron S. Field, Mauro Corrado, Fabian Haessler, Chao Wang, Yaarub Musa, Lena Schimmelpfennig, Lea Flachsmann, Gerhard Mittler, Nir Yosef, Vijay K. Kuchroo, Joerg M. Buescher, Stefan Balabanov, Edward J. Pearce, Douglas R. Green, Erika L. Pearce^*

Published graphical abstract showing polyamine-driven eIF5A hypusination regulating mitochondrial TCA-cycle and respiratory-chain protein expression and alternative macrophage activation.

Cell Metabolism2019

Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation

Daniel J. Puleston, Michael D. Buck, Ramon I. Klein Geltink, …, Edward J. Pearce, Stefan Balabanov, Erika L. Pearce^*

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Daniel J. Puleston, Michael D. Buck, Ramon I. Klein Geltink, Ryan L. Kyle, George Caputa, David O'Sullivan, Alanna M. Cameron, Angela Castoldi, Yaarub Musa, Agnieszka M. Kabat, Ying Zhang, Lea J. Flachsmann, Cameron S. Field, Annette E. Patterson, Stefanie Scherer, Francesca Alfei, Francesc Baixauli, S. Kyle Austin, Beth Kelly, Mai Matsushita, Jonathan D. Curtis, Katarzyna M. Grzes, Matteo Villa, Mauro Corrado, David E. Sanin, Jing Qiu, Nora Pällman, Katelyn Paz, Maria Elena Maccari, Bruce R. Blazar, Gerhard Mittler, Joerg M. Buescher, Dietmar Zehn, Sabine Rospert, Edward J. Pearce, Stefan Balabanov, Erika L. Pearce^*

More publications

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2026

Nature Reviews Immunology2026

Glycolytic capacity instructs tumour vasculature and response to immunotherapy

Giang Pham, Daniel J. Puleston^*

Nature Communications2026

Cellular hallmarks and aging clock of the human lung parenchyma

Ke Xu, Grace S. Kim, Atharva Bhagwat, Sepideh E. Meimand, Vignesh V. Venkat, Giang Pham, Jing Zhang, Jamshid Abdul-Ghafar, Mohamad D. Bairakdar, Komal Dolasia, Isha R. Sahasrabudhe, Alter Klausner, Yijia Chen, Thinh T. Nguyen, Bruno Giotti, Rachel Brody, So-Jin Kim, Jaymin J. Kathiriya, Daniel J. Puleston, Patty J. Lee, Alexander M. Tsankov^*

bioRxiv2026Preprint

Tetherin enforces an immunometabolic checkpoint that coordinates glycolytic and interferon signaling in adipocytes

Chung Hwan Cho, YoungUk Jang, Aidan Warnock, Ramazan Yildiz, Jing Jhang, Kajal Davi, Niki F. Brisnovali, Victoria Huhn, Peng Wang, Romina Bevaqua, Leigh Goedeke, Michael A. Schotsaert, Mirela Berisa, Daniel Puleston, Prashant Rajbhandari^*

Cancer Discovery2026

Ontogeny Dictates Oncogenic Potential, Lineage Hierarchy, and Therapy Response in Pediatric Leukemia

Ke Wang, Shayan Saniei, Nikita Poddar, Isabella G. Martinez, Clifford Chao, Subrina Autar, Persephone Fiore, Saul Carcamo, Meghana Sreenath, Jack H. Peplinski, Rhonda E. Ries, Anna Huo-Chang Mei, Noshin Azra Rahman, Levan Mekerishvili, Miguel Quijada-Álamo, Grace Freed, Mimi Zhang, Katherine Lachman, Zayna Diaz, Manuel M. Gonzalez, Jing Zhang, Giang Pham, Dan Filipescu, Mirela Berisa, Tommaso Balestra, Noelle Wheeler, Julie A. Reisz, Angelo D'Alessandro, Daniel J. Puleston, Emily Bernstein, Jerry E. Chipuk, Mark Wunderlich, Sarah K. Tasian, Bridget K. Marcellino, Ian A. Glass, BDRL, Christopher M. Sturgeon, Dan A. Landau, Zhihong Chen, Eirini P. Papapetrou, Franco Izzo, Soheil Meshinchi, Dan Hasson, Elvin Wagenblast^*

2025

Nature Aging2025

Restoring resident tissue macrophages to combat aging and cancer

Matthew D. Park, Nader Yatim, Jing Zhang, Byuri Angela Cho, Seong-Keun Yoo, Maximilian M. Schaefer, Diego Chowell, Daniel J. Puleston, Miriam Merad^*

Nature Reviews Cancer2025

Dendritic cell maturation in cancer

Chang Yoon Moon, Meriem Belabed, Matthew D. Park, Raphaël Mattiuz, Daniel Puleston, Miriam Merad^*

bioRxiv2025Preprint

Ontogeny Dictates Oncogenic Potential, Lineage Hierarchy, and Therapy Response in Pediatric Leukemia

Ke Wang, Shayan Saniei, Nikita Poddar, Subrina Autar, Saul Carcamo, Meghana Sreenath, Jack H. Peplinski, Rhonda E. Ries, Isabella G. Martinez, Clifford Chao, Anna Huo-Chang Mei, Noshin Rahman, Levan Mekerishvili, Miguel Quijada-Álamo, Grace Freed, Mimi Zhang, Katherine Lachman, Zayna Diaz, Manuel M. Gonzalez, Jing Zhang, Giang Pham, Dan Filipescu, Mirela Berisa, Tommaso Balestra, Julie A. Reisz, Angelo D'Alessandro, Daniel J. Puleston, Emily Bernstein, Jerry E. Chipuk, Mark Wunderlich, Sarah K. Tasian, Bridget K. Marcellino, Ian A. Glass, BDRL, Christopher M. Sturgeon, Dan A. Landau, Zhihong Chen, Eirini P. Papapetrou, Franco Izzo, Soheil Meshinchi, Dan Hasson, Elvin Wagenblast^*

Preprint of the 2026 published article.

Artificial Organs2025

Normothermic machine perfusion of explanted livers: Exploratory study of an alternative translational model for end-stage liver disease

Rachel Todd, L. Leonie van Leeuwen, Matthew Holzner, Leona Kim-Schluger, Maria Isabel Fiel, Daniel Puleston, Sander S. Florman, M. Zeeshan Akhtar^*

Nature Immunology2025

Cholesterol mobilization regulates dendritic cell maturation and the immunogenic response to cancer

Meriem Belabed, Matthew D. Park, Cédric M. Blouin, Sreekumar Balan, Chang Y. Moon, Grace Freed, Miguel Quijada-Álamo, Ante Peros, Raphaël Mattiuz, Amanda M. Reid, Nader Yatim, Jesse Boumelha, Camillia S. Azimi, Nelson M. LaMarche, Leanna Troncoso, Angelo Amabile, Jessica Le Berichel, Steven T. Chen, C. Matthias Wilk, Brian D. Brown, Kristen J. Radford, Sourav Ghosh, Carla V. Rothlin, Laurent Yvan-Charvet, Thomas U. Marron, Daniel J. Puleston, Elvin Wagenblast, Nina Bhardwaj, Christophe Lamaze, Miriam Merad^*

2024

Nature Communications2024

Prostaglandin E2 controls the metabolic adaptation of T cells to the intestinal microenvironment

Matteo Villa^*, David E. Sanin, Petya Apostolova, Mauro Corrado, Agnieszka M. Kabat, Carmine Cristinzio, Annamaria Regina, Gustavo E. Carrizo, Nisha Rana, Michal A. Stanczak, Francesc Baixauli, Katarzyna M. Grzes, Jovana Cupovic, Francesca Solagna, Alexandra Hackl, Anna-Maria Globig, Fabian Hässler, Daniel J. Puleston, Beth Kelly, Nina Cabezas-Wallscheid, Peter Hasselblatt, Bertram Bengsch, Robert Zeiser, Sagar, Joerg M. Buescher, Edward J. Pearce, Erika L. Pearce^*

2023

Nature2023

A metabolic vulnerability of pancreatic cancer

Daniel J. Puleston^*

bioRxiv2023Preprint

Prostaglandin E2 controls the metabolic adaptation of T cells to the intestinal microenvironment

Preprint of the 2024 published article.

Nature Immunology2023

Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development

Stefanie Scherer, Susanne G. Oberle, Kristiyan Kanev, Ann-Katrin Gerullis, Ming Wu, Gustavo P. de Almeida, Daniel J. Puleston, Francesc Baixauli, Lilian Aly, Alessandro Greco, Tamar Nizharadze, Nils B. Becker, Madlaina V. Hoesslin, Lara V. Donhauser, Jacqueline Berner, Talyn Chu, Hayley A. McNamara, Zeynep Esencan, Patrick Roelli, Christine Wurmser, Ingo Kleiter, Maria J. G. T. Vehreschild, Christoph A. Mayer, Percy Knolle, Martin Klingenspor, Valeria Fumagalli, Matteo Iannacone, Martin Prlic, Thomas Korn, Erika L. Pearce, Thomas Höfer, Anna M. Schulz^*, Dietmar Zehn^*

Nature Immunology2023

Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function

Joy Edwards-Hicks, Petya Apostolova, Joerg M. Buescher, Hannes Maib, Michal A. Stanczak, Mauro Corrado, Ramon I. Klein Geltink, Maria Elena Maccari, Matteo Villa, Gustavo E. Carrizo, David E. Sanin, Francesc Baixauli, Beth Kelly, Jonathan D. Curtis, Fabian Haessler, Annette Patterson, Cameron S. Field, George Caputa, Ryan L. Kyle, Melanie Soballa, Minsun Cha, Harry Paul, Jacob Martin, Katarzyna M. Grzes, Lea Flachsmann, Michael Mitterer, Liang Zhao, Frances Winkler, David Ali Rafei-Shamsabadi, Frank Meiss, Bertram Bengsch, Robert Zeiser, Daniel J. Puleston, David O'Sullivan, Edward J. Pearce, Erika L. Pearce^*

Cell Metabolism2023

Metabolic adaptation supports enhanced macrophage efferocytosis in limited-oxygen environments

Ya-Ting Wang, Alissa J. Trzeciak, Waleska Saitz Rojas, Pedro Saavedra, Yan-Ting Chen, Rachel Chirayil, Jon Iker Etchegaray, Christopher D. Lucas, Daniel J. Puleston, Kayvan R. Keshari, Justin S. A. Perry^*

2022

Nature2022

An LKB1-mitochondria axis controls TH17 effector function

Francesc Baixauli, Klara Piletic, Daniel J. Puleston, Matteo Villa, Cameron S. Field, Lea J. Flachsmann, Andrea Quintana, Nisha Rana, Joy Edwards-Hicks, Mai Matsushita, Michal A. Stanczak, Katarzyna M. Grzes, Agnieszka M. Kabat, Mario Fabri, George Caputa, Beth Kelly, Mauro Corrado, Yaarub Musa, Katarzyna J. Duda, Gerhard Mittler, David O'Sullivan, Hiromi Sesaki, Thomas Jenuwein, Joerg M. Buescher, Edward J. Pearce, David E. Sanin, Erika L. Pearce^*

Nature Communications2022

Mapping autophagosome contents identifies interleukin-7 receptor-α as a key cargo modulating CD4+ T cell proliferation

Dingxi Zhou, Mariana Borsa, Daniel J. Puleston, Susanne Zellner, Jesusa Capera, Sharon Sanderson, Martina Schifferer, Svenja S. Hester, Xin Ge, Roman Fischer, Luke Jostins, Christian Behrends, Ghada Alsaleh, Anna Katharina Simon^*

2021

Cell2021

Metabolic modeling of single Th17 cells reveals regulators of autoimmunity

Allon Wagner, Chao Wang^*, Johannes Fessler, David DeTomaso, Julian Avila-Pacheco, James Kaminski, Sarah Zaghouani, Elena Christian, Pratiksha Thakore, Brandon Schellhaass, Elliot Akama-Garren, Kerry Pierce, Vasundhara Singh, Noga Ron-Harel, Vivian Paraskevi Douglas, Lloyd Bod, Alexandra Schnell, Daniel Puleston, Raymond A. Sobel, Marcia Haigis, Erika L. Pearce, Manoocher Soleimani, Clary Clish, Aviv Regev, Vijay K. Kuchroo^*, Nir Yosef^*

PNAS2021

Fever supports CD8+ effector T cell responses by promoting mitochondrial translation

David O'Sullivan, Michal A. Stanczak, Matteo Villa, Franziska M. Uhl, Mauro Corrado, Ramon I. Klein Geltink, David E. Sanin, Petya Apostolova, Nisha Rana, Joy Edwards-Hicks, Katarzyna M. Grzes, Agnieszka M. Kabat, Ryan L. Kyle, Mario Fabri, Jonathan D. Curtis, Michael D. Buck, Annette E. Patterson, Annamaria Regina, Cameron S. Field, Francesc Baixauli, Daniel J. Puleston, Edward J. Pearce, Robert Zeiser, Erika L. Pearce^*

2020

Nature Metabolism2020

Metabolic conditioning of CD8+ effector T cells for adoptive cell therapy

Ramon I. Klein Geltink, Joy Edwards-Hicks, Petya Apostolova, David O'Sullivan, David E. Sanin, Annette E. Patterson, Daniel J. Puleston, Nina A. M. Ligthart, Joerg M. Buescher, Katarzyna M. Grzes, Agnieszka M. Kabat, Michal Stanczak, Jonathan D. Curtis, Fabian Hässler, Franziska M. Uhl, Mario Fabri, Robert Zeiser, Edward J. Pearce, Erika L. Pearce^*

Cell Metabolism2020

Appetite for Arginine: Metabolic Control of Macrophage Hunger

Daniel J. Puleston, Erika L. Pearce^*

Cell Metabolism2020

Mitochondrial Integrity Regulated by Lipid Metabolism Is a Cell-Intrinsic Checkpoint for Treg Suppressive Function

Cameron S. Field, Francesc Baixauli, Ryan L. Kyle, Daniel J. Puleston, Alanna M. Cameron, David E. Sanin, Keli L. Hippen, Michael Loschi, Govindarajan Thangavelu, Mauro Corrado, Joy Edwards-Hicks, Katarzyna M. Grzes, Edward J. Pearce, Bruce R. Blazar, Erika L. Pearce^*

2019

Nature Immunology2019

Inflammatory macrophage dependence on NAD+ salvage is a consequence of reactive oxygen species-mediated DNA damage

Alanna M. Cameron, Angela Castoldi, David E. Sanin, Lea J. Flachsmann, Cameron S. Field, Daniel J. Puleston, Ryan L. Kyle, Annette E. Patterson, Fabian Hässler, Joerg M. Buescher, Beth Kelly, Erika L. Pearce, Edward J. Pearce^*

2017

Cell Metabolism2017

Ancillary Activity: Beyond Core Metabolism in Immune Cells

Daniel J. Puleston, Matteo Villa, Erika L. Pearce^*

2016

Trends in Molecular Medicine2016

Autophagy and Immune Senescence

Hanlin Zhang, Daniel J. Puleston, Anna Katharina Simon^*

2015

Cold Spring Harbor Protocols2015

Techniques for the Detection of Autophagy in Primary Mammalian Cells

Daniel Puleston, Kanchan Phadwal, Alexander Scarth Watson, Elizabeth J. Soilleux, Suganthi Chittaranjan, Svetlana Bortnik, Sharon M. Gorski, Nicholas Ktistakis, Anna Katharina Simon^*

Cold Spring Harbor Protocols2015

Detection of Mitochondrial Mass, Damage, and Reactive Oxygen Species by Flow Cytometry

Daniel Puleston^*

Cell Death Discovery2015

Autophagy limits proliferation and glycolytic metabolism in acute myeloid leukemia

Alexander S. Watson, Thomas Riffelmacher, Amanda Stranks, Owen Williams, Jasper De Boer, Kelvin Cain, Marion MacFarlane, Joanna McGouran, Benedikt Kessler, Shivani Khandwala, Onima Chowdhury, Daniel Puleston, Kanchan Phadwal, Monika Mortensen, David Ferguson, Elizabeth Soilleux, Petter Woll, Sten Eirik W. Jacobsen, Anna Katharina Simon^*

Journal of Innate Immunity2015

Autophagy Controls Acquisition of Aging Features in Macrophages

Amanda J. Stranks, Anne Louise Hansen, Isabel Panse, Monika Mortensen, David J. P. Ferguson, Daniel J. Puleston, Kevin Shenderov, Alexander Scarth Watson, Marc Veldhoen, Kanchan Phadwal, Vincenzo Cerundolo, Anna Katharina Simon^*

Autophagy2015

Mitochondrial damage contributes to Pseudomonas aeruginosa activation of the inflammasome and is downregulated by autophagy

Majid Sakhi Jabir, Lee Hopkins, Neil D. Ritchie, Ihsan Ullah, Hannah K. Bayes, Dong Li, Panagiotis Tourlomousis, Alison Lupton, Daniel Puleston, Anna Katharina Simon, Clare Bryant, Thomas J. Evans^*

2014

PNAS2014

Caspase-1 cleavage of the TLR adaptor TRIF inhibits autophagy and β-interferon production during Pseudomonas aeruginosa infection

Majid Sakhi Jabir, Neil D. Ritchie, Dong Li, Hannah K. Bayes, Panagiotis Tourlomousis, Daniel Puleston, Alison Lupton, Lee Hopkins, Anna Katharina Simon, Clare Bryant, Thomas J. Evans^*

Immunology2014

Autophagy in the immune system

Daniel J. Puleston, Anna Katharina Simon^*

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